Growth rate metrics
“Growth rate (GR) metrics”[1][2] were developed as an alternative to traditional drug sensitivity/resistance metrics (such as IC50 and Emax), which can be confounded when cells continued to grow during the assay.
GR metrics measure the effect of a perturbation on the growth rate of a cell population rather than on the percent viability. This creates a transformed “dose-response” curve with new summary metrics (GR50, GRmax, etc.). These new curves and metrics can measure growth rate effects due to drug treatment and can be applied to other perturbations, for example on genetic manipulation or on cells plated at different densities.
On the following pages, you can learn more about the computational methdos used in the classical GR method and the new addition of static and toxic growth rate metrics.
Growth rate metrics can be calculated in two ways:
- The GR calculator provides a web interface for computation of GR metrics.
- For offline computation, analysis, and visualization, we provide an R package available on Bioconductor: GRmetrics.