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4. areaFilter: Cell segmentation errors introduce significant error into image-derived, single-cell data. In this module, users assign lower and upper cutoffs on cell segmentation area of cells comprising each tissue as calculated by the quantification module in MCMICRO to remove under- and over-segmented cells. After gate placement, users can visualize selected cells in their corresponding tissue by clicking the Plot Points button beneath the histogram. Closing the Napari window causes the program to apply the current cutoffs and proceed to the next tissue for segmentation area cutoff assignment.

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