USP32 Data Analysis

HGNC Gene Name
ubiquitin specific peptidase 32
HGNC Gene Symbol
USP32
Identifiers
hgnc:19143 NCBIGene:84669 uniprot:Q8NFA0
Orthologs
mgi:2144475 rgd:1306657
INDRA Statements
deubiquitinations all statements
Pathway Commons
Search for USP32
Number of Papers
21 retrieved on 2022-05-22

DepMap Analysis

The Dependency Map (DepMap) is a genome-wide pooled CRISPR-Cas9 knockout proliferation screen conducted in more than 700 cancer cell lines spanning many different tumor lineages. Each cell line in the DepMap contains a unique barcode, and each gene knockout is assigned a “dependency score” on a per cell-line basis which quantifies the rate of CRISPR-Cas9 guide drop. It has been found that proteins with similar DepMap scores across cell lines, a phenomenon known as co-dependent genes, have closely related biological functions. This can include activity in the same or parallel pathways or membership in the same protein complex or the same pathway.

We identified the strongest seven co-dependent genes (“Symbol”) for DUBs and ran GO enrichment analysis. We used Biogrid, IntAct, and Pathway Commons PPIDs, and the NURSA protein-protein interaction databases (PPIDs) to determine whether co-dependent genes interact with one another. The “Evidence” column contains the PPIDs in which the interaction appears as well as whether there is support for the association by an INDRA statement. As another approach to identify potential interactors, we looked at proteomics data from the Broad Institute's Cancer Cell Line Encyclopedia (CCLE) for proteins whose expression across ~375 cell lines strongly correlated with the abundance of each DUB; it has previously been observed that proteins in the same complex are frequently significantly co-expressed. The correlations and associated p-values in the CCLE proteomics dataset are provided. And, we determined whether co-dependent genes yield similar transcriptomic signatures in the Broad Institute's Connectivity Map (CMap). A CMap score greater than 90 is considered significantly similar.

DepMap Correlations

Symbol Name DepMap Correlation Evidence CCLE Correlation CCLE Z-score CCLE p-value (adj) CCLE Significant CMAP Score CMAP Type
TNRC6C trinucleotide repeat containing adaptor 6C 0.202 0.09 0.42 2.82e-01
VPS54 VPS54 subunit of GARP complex 0.199 0.31 1.63 1.87e-04
EMC3 ER membrane protein complex subunit 3 0.192 0.12 0.56 7.13e-02
RAB6A RAB6A, member RAS oncogene family 0.192 0.04 0.14 5.77e-01
VPS52 VPS52 subunit of GARP complex 0.191 0.34 1.78 1.96e-09
CNFN cornifelin -0.188
ARL15 ADP ribosylation factor like GTPase 15 -0.187 -0.02 -0.22 7.74e-01

Dependency GO Term Enrichment

Gene set enrichment analysis was done on the genes correlated with USP32using the terms from Gene Ontology and gene sets derived from the Gene Ontology Annotations database via MSigDB.

Using the biological processes and other Gene Ontology terms from well characterized DUBs as a positive control, several gene set enrichment analyses were considered. Threshold-less methods like GSEA had relatively poor results. Over-representation analysis with a threshold of of the top 7 highest absolute value Dependency Map correlations yielded the best results and is reported below.

GO Identifier GO Name GO Type p-value p-value (adj.) q-value
GO:0000938 GARP complex Cellular Component 1.17e-06 1.01e-04 4.79e-05
GO:0042147 retrograde transport, endosome to Golgi Biological Process 3.11e-06 2.70e-04 5.21e-05
GO:0032588 trans-Golgi network membrane Cellular Component 3.81e-06 3.31e-04 5.21e-05
GO:0006896 Golgi to vacuole transport Biological Process 1.06e-05 9.22e-04 1.09e-04
GO:0016482 cytosolic transport Biological Process 1.86e-05 1.62e-03 1.53e-04
GO:0016197 endosomal transport Biological Process 5.27e-05 4.59e-03 3.61e-04
GO:0005802 trans-Golgi network Cellular Component 6.33e-05 5.51e-03 3.71e-04
GO:0048193 Golgi vesicle transport Biological Process 2.33e-04 2.03e-02 1.06e-03
GO:0031984 organelle subcompartment Cellular Component 2.59e-04 2.25e-02 1.06e-03
GO:0019905 syntaxin binding Molecular Function 2.86e-04 2.49e-02 1.07e-03

Literature Mining

INDRA was used to automatically assemble known mechanisms related to USP32 from literature and knowledge bases. The first section shows only DUB activity and the second shows all other results.

Deubiquitinase Activity

Other Statements

psp cbn pc bel_lc signor biogrid lincs_drug tas hprd trrust ctd vhn pe drugbank omnipath conib crog dgi | rlimsp isi tees geneways eidos trips medscan sparser reach
MEIS1 affects USP32
3 |
MEIS1 decreases the amount of USP32. 3 / 3
3 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
2 |
Hsa-miR-93-5p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available
2 |
Hsa-miR-519d-3p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available
2 |
Hsa-miR-20b-5p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available
2 |
Hsa-miR-20a-5p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available
2 |
Hsa-miR-17-5p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available
2 |
Hsa-miR-106b-5p decreases the amount of USP32. 2 / 2
2 |

biopax:mirtarbase
No evidence text available

biopax:mirtarbase
No evidence text available

reach
In the present study, we reported that knockdown or depletion of USP32 significantly inhibited GC cell proliferation and migration in vitro and in vivo, indicating that USP32 functions as an oncogene in GC.

reach
Further, stable silencing of USP32 expression reduced proliferation and migration in the ERalpha positive MCF-7 cell line [XREF_BIBR].
ELK1 affects USP32
2 |
ELK1 decreases the amount of USP32. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
Tungsten affects USP32
1 |
Tungsten decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available

ctd
No evidence text available
Topotecan affects USP32
1 |
Topotecan decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Raloxifene hydrochloride decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Progesterone increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Potassium chromate decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available
Paracetamol affects USP32
1 |
Paracetamol decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Oxaliplatin affects USP32
1 |
Oxaliplatin decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Methamphetamine decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Lead atom affects USP32
1 |
Lead atom increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Ionomycin affects USP32
1 |
Ionomycin decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Hsa-miR-7156-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-7110-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6873-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6833-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6817-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-548ah-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-526b-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-519c-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-519b-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-519a-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4796-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4768-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4753-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4696 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-454-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4295 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4273 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3666 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3609 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-301b-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-301a-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-2117 decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-18a-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-130b-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-130b-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-130a-3p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-124-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-106a-5p decreases the amount of USP32. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hexabromocyclododecane decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Fulvestrant affects USP32
1 |
Fulvestrant decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Dibutyl phthalate increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Cyclosporin A increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Cupric oxide increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Copper(II) sulfate decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Cisplatin affects USP32
1 |
Cisplatin decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Cadmium dichloride decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available
1 |
Aluminium atom decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
Aflatoxin B1 increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Afimoxifene affects USP32
1 |
Afimoxifene decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
Acrylamide affects USP32
1 |
Acrylamide decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
USP32 affects transport
| 1
| 1

reach
Loss of USP32 inhibits late endosome (LE) transport and recycling of LE cargos, resulting in dispersion and swelling of the late compartment.
USP32 affects cell cycle
| 1
| 1

reach
Furthermore, USP32 silencing arrested cell cycle progression at G0/G1 phase via decreasing CDK4 and Cyclin D1 complex and elevating p21.
| 1

reach
In addition, downregulation of USP32 significantly induced cell apoptosis by activating cleaved caspase-3 and cleaved PARP, as well as inhibiting cell invasiveness via altering epithelial mesenchymal transition expression.
USP32 affects SMAD2
| 1
USP32 increases the amount of SMAD2. 1 / 1
| 1

reach
Mechanistically, USP32 silencing caused a decrease in the expression of SMAD2, which resulted in the inhibitory effects of GC cells on growth, motility, and chemoresistance to cisplatin.
USP32 affects PARP1
| 1
USP32 activates PARP1. 1 / 1
| 1

reach
In addition, downregulation of USP32 significantly induced cell apoptosis by activating cleaved caspase-3 and cleaved PARP, as well as inhibiting cell invasiveness via altering epithelial mesenchymal transition expression.

reach
In addition, downregulation of USP32 significantly induced cell apoptosis by activating cleaved caspase-3 and cleaved PARP, as well as inhibiting cell invasiveness via altering epithelial mesenchymal transition expression.
USP32 affects FUT3
| 1
USP32 activates FUT3. 1 / 1
| 1

reach
Loss of USP32 inhibits late endosome (LE) transport and recycling of LE cargos, resulting in dispersion and swelling of the late compartment.
USP32 affects CASP3
| 1
USP32 activates CASP3. 1 / 1
| 1

reach
In addition, downregulation of USP32 significantly induced cell apoptosis by activating cleaved caspase-3 and cleaved PARP, as well as inhibiting cell invasiveness via altering epithelial mesenchymal transition expression.
TCF3 affects USP32
1 |
TCF3 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
SRY affects USP32
1 |
SRY decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
SP1 affects USP32
1 |
SP1 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
SOX5 affects USP32
1 |
SOX5 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
RREB1 affects USP32
1 |
RREB1 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
REPIN1 affects USP32
1 |
REPIN1 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
1 |
Plant Extracts increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
POU3F2 affects USP32
1 |
POU3F2 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
PAX4 affects USP32
1 |
PAX4 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
MYB affects USP32
1 |
MYB decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
LEF1 affects USP32
1 |
LEF1 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
IRF1 affects USP32
1 |
IRF1 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
HOXA9 affects USP32
1 |
HOXA9 decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
CEBPA affects USP32
1 |
CEBPA decreases the amount of USP32. 1 / 1
1 |

biopax:msigdb
No evidence text available
1 |
C60 fullerene increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |
BEP protocol decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
2-hydroxypropanoic acid decreases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available
1 |
17beta-estradiol increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1,3-dinitrobenzene increases the amount of USP32. 1 / 1
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available