PRPF8 Data Analysis

HGNC Gene Name: pre-mRNA processing factor 8
HGNC Gene Symbol: PRPF8
Identifiers: hgnc:17340 NCBIGene:10594 uniprot:Q6P2Q9
Orthologs: mgi:2179381 rgd:1305467
INDRA Statements: deubiquitinations all statements
Pathway Commons: Search for PRPF8
Number of Papers: 152 retrieved on 2023-02-19

DepMap Analysis

The Dependency Map (DepMap) is a genome-wide pooled CRISPR-Cas9 knockout proliferation screen conducted in more than 700 cancer cell lines spanning many different tumor lineages. Each cell line in the DepMap contains a unique barcode, and each gene knockout is assigned a “dependency score” on a per cell-line basis which quantifies the rate of CRISPR-Cas9 guide drop. It has been found that proteins with similar DepMap scores across cell lines, a phenomenon known as co-dependent genes, have closely related biological functions. This can include activity in the same or parallel pathways or membership in the same protein complex or the same pathway.

We identified the strongest seven co-dependent genes (“Symbol”) for DUBs and ran GO enrichment analysis. We used Biogrid, IntAct, and Pathway Commons PPIDs, and the NURSA protein-protein interaction databases (PPIDs) to determine whether co-dependent genes interact with one another. The “Evidence” column contains the PPIDs in which the interaction appears as well as whether there is support for the association by an INDRA statement. As another approach to identify potential interactors, we looked at proteomics data from the Broad Institute's Cancer Cell Line Encyclopedia (CCLE) for proteins whose expression across ~375 cell lines strongly correlated with the abundance of each DUB; it has previously been observed that proteins in the same complex are frequently significantly co-expressed. The correlations and associated p-values in the CCLE proteomics dataset are provided. And, we determined whether co-dependent genes yield similar transcriptomic signatures in the Broad Institute's Connectivity Map (CMap). A CMap score greater than 90 is considered significantly similar.

DepMap Correlations

Symbol	Name	DepMap Correlation	Evidence	CCLE Correlation	CCLE Z-score	CCLE p-value (adj)
HNRNPK	heterogeneous nuclear ribonucleoprotein K	0.472	INDRA (2) Reactome (4)	-0.45	-2.57	8.90e-09
RAN	RAN, member RAS oncogene family	0.47	Reactome (1)	-0.31	-1.80	1.03e-08
PSMD7	proteasome 26S subunit, non-ATPase 7	0.44	Reactome (1)	0.04	0.11	6.18e-01
SNRNP200	small nuclear ribonucleoprotein U5 subunit 200	0.42	BioGRID IntAct INDRA (13) Reactome (5)	0.93	5.06	4.62e-166
SF3A1	splicing factor 3a subunit 1	0.418	BioGRID INDRA (1) Reactome (4)	-0.06	-0.42	3.58e-01
PHF5A	PHD finger protein 5A	0.41	Reactome (4)	-0.34	-1.94	4.13e-10
SNRPB	small nuclear ribonucleoprotein polypeptides B and B1	0.409	BioGRID INDRA (1) Reactome (5)	-0.05	-0.34	5.13e-01

Dependency GO Term Enrichment

Gene set enrichment analysis was done on the genes correlated with PRPF8using the terms from Gene Ontology and gene sets derived from the Gene Ontology Annotations database via MSigDB.

Using the biological processes and other Gene Ontology terms from well characterized DUBs as a positive control, several gene set enrichment analyses were considered. Threshold-less methods like GSEA had relatively poor results. Over-representation analysis with a threshold of of the top 7 highest absolute value Dependency Map correlations yielded the best results and is reported below.

GO Identifier	GO Name	GO Type	p-value	p-value (adj.)	q-value
GO:0005681	spliceosomal complex	Cellular Component	1.42e-09	3.71e-07	1.84e-07
GO:0071013	catalytic step 2 spliceosome	Cellular Component	1.43e-08	3.73e-06	9.24e-07
GO:0120114	Sm-like protein family complex	Cellular Component	3.97e-08	1.04e-05	1.61e-06
GO:0005686	U2 snRNP	Cellular Component	6.20e-08	1.62e-05	1.61e-06
GO:0000375	RNA splicing, via transesterification reactions	Biological Process	6.21e-08	1.62e-05	1.61e-06
GO:0008380	RNA splicing	Biological Process	1.75e-07	4.55e-05	3.77e-06
GO:0071011	precatalytic spliceosome	Cellular Component	4.05e-07	1.06e-04	7.48e-06
GO:0005684	U2-type spliceosomal complex	Cellular Component	2.79e-06	7.28e-04	4.51e-05
GO:0071010	prespliceosome	Cellular Component	1.40e-05	3.65e-03	1.81e-04
GO:0022613	ribonucleoprotein complex biogenesis	Biological Process	1.40e-05	3.65e-03	1.81e-04
GO:0005682	U5 snRNP	Cellular Component	2.69e-05	7.01e-03	3.16e-04
GO:0005689	U12-type spliceosomal complex	Cellular Component	4.08e-05	1.06e-02	4.40e-04
GO:0097526	spliceosomal tri-snRNP complex	Cellular Component	1.05e-04	2.73e-02	1.04e-03
GO:0071826	ribonucleoprotein complex subunit organization	Biological Process	1.15e-04	2.99e-02	1.06e-03

Transcriptomics

The following table shows the significantly differentially expressed genes after knocking out PRPF8 using CRISPR-Cas9.

Knockout Differential Expression

Symbol	Name	log₂-fold-change	p-value	p-value (adj.)
HMGB2	high mobility group box 2	7.40e-01	2.26e-14	6.37e-11
ANKRD1	ankyrin repeat domain 1	6.05e-01	9.95e-14	1.40e-10
C4BPB	complement component 4 binding protein beta	1.03e+00	1.06e-11	9.98e-09
MT-ND2	mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 2	4.02e-01	3.65e-10	2.57e-07
HMGB3	high mobility group box 3	4.71e-01	1.52e-08	8.59e-06
PDRG1	p53 and DNA damage regulated 1	7.56e-01	6.25e-08	2.93e-05
SAT1	spermidine/spermine N1-acetyltransferase 1	5.66e-01	8.44e-08	3.40e-05
G0S2	G0/G1 switch 2	-4.83e-01	2.10e-07	7.41e-05
MT-ATP8	mitochondrially encoded ATP synthase membrane subunit 8	6.96e-01	3.60e-07	1.13e-04
MRPS16	mitochondrial ribosomal protein S16	-5.26e-01	1.95e-06	5.49e-04
SRP9	signal recognition particle 9	-4.61e-01	2.85e-06	7.30e-04
SART3	spliceosome associated factor 3, U4/U6 recycling protein	7.08e-01	7.35e-06	1.72e-03
EIF4A2	eukaryotic translation initiation factor 4A2	4.92e-01	1.11e-05	2.08e-03
RND3	Rho family GTPase 3	6.65e-01	1.07e-05	2.08e-03
SLC25A5	solute carrier family 25 member 5	-2.99e-01	1.10e-05	2.08e-03
PSMB4	proteasome 20S subunit beta 4	-4.28e-01	1.73e-05	3.04e-03
UQCC2	ubiquinol-cytochrome c reductase complex assembly factor 2	-4.03e-01	1.94e-05	3.21e-03
UQCRH	ubiquinol-cytochrome c reductase hinge protein	3.22e-01	2.09e-05	3.26e-03
FDPS	farnesyl diphosphate synthase	-4.42e-01	2.31e-05	3.27e-03
WDR83OS	WD repeat domain 83 opposite strand	2.93e-01	2.32e-05	3.27e-03
EIF3K	eukaryotic translation initiation factor 3 subunit K	-4.23e-01	3.86e-05	5.11e-03
MT-ND1	mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1	2.56e-01	3.99e-05	5.11e-03
ARF1	ADP ribosylation factor 1	4.08e-01	4.50e-05	5.28e-03
RBM39	RNA binding motif protein 39	3.77e-01	4.32e-05	5.28e-03
C4orf3	chromosome 4 open reading frame 3	3.42e-01	4.90e-05	5.52e-03
JMJD1C	jumonji domain containing 1C	6.13e-01	5.74e-05	6.22e-03
FADS1	fatty acid desaturase 1	-5.68e-01	6.01e-05	6.27e-03
MTCO1P12	MT-CO1 pseudogene 12	3.37e-01	6.65e-05	6.69e-03
VPS29	VPS29 retromer complex component	-3.76e-01	8.36e-05	8.12e-03
AKR1B1	aldo-keto reductase family 1 member B	3.44e-01	1.21e-04	1.12e-02
ARL6IP4	ADP ribosylation factor like GTPase 6 interacting protein 4	-6.08e-01	1.24e-04	1.12e-02
BDNF	brain derived neurotrophic factor	6.24e-01	1.57e-04	1.34e-02
DDX55	DEAD-box helicase 55	5.58e-01	1.56e-04	1.34e-02
SF3B1	splicing factor 3b subunit 1	4.63e-01	2.06e-04	1.70e-02
CBX5	chromobox 5	4.94e-01	2.12e-04	1.71e-02
TM4SF1	transmembrane 4 L six family member 1	3.54e-01	2.19e-04	1.72e-02
CKS2	CDC28 protein kinase regulatory subunit 2	3.60e-01	2.70e-04	2.05e-02
UQCRC1	ubiquinol-cytochrome c reductase core protein 1	-3.82e-01	2.81e-04	2.08e-02
EIF3B	eukaryotic translation initiation factor 3 subunit B	-6.68e-01	3.18e-04	2.30e-02
VDAC2	voltage dependent anion channel 2	3.30e-01	3.26e-04	2.30e-02
ANXA3	annexin A3	-4.55e-01	3.43e-04	2.30e-02
LPXN	leupaxin	-5.43e-01	3.40e-04	2.30e-02
SLC3A2	solute carrier family 3 member 2	5.47e-01	3.65e-04	2.39e-02
RRM2	ribonucleotide reductase regulatory subunit M2	-5.45e-01	3.83e-04	2.40e-02
VIM	vimentin	-3.48e-01	3.84e-04	2.40e-02
MT-CO1	mitochondrially encoded cytochrome c oxidase I	3.56e-01	4.13e-04	2.49e-02
RNASEH2A	ribonuclease H2 subunit A	-3.78e-01	4.16e-04	2.49e-02
PIGN	phosphatidylinositol glycan anchor biosynthesis class N	5.48e-01	5.24e-04	3.07e-02
MRPL24	mitochondrial ribosomal protein L24	-3.90e-01	5.58e-04	3.12e-02
PRPF8	pre-mRNA processing factor 8	-6.57e-01	5.47e-04	3.12e-02
SRGN	serglycin	4.40e-01	5.65e-04	3.12e-02
DNAJA1	DnaJ heat shock protein family (Hsp40) member A1	2.62e-01	6.16e-04	3.19e-02
KRT19	keratin 19	-3.19e-01	6.25e-04	3.19e-02
MTCH2	mitochondrial carrier 2	-3.32e-01	6.34e-04	3.19e-02
POLR1C	RNA polymerase I and III subunit C	4.43e-01	6.18e-04	3.19e-02
SRSF6	serine and arginine rich splicing factor 6	-4.29e-01	6.11e-04	3.19e-02
CLPP	caseinolytic mitochondrial matrix peptidase proteolytic subunit	-5.77e-01	6.86e-04	3.28e-02
SEC22B	SEC22 homolog B, vesicle trafficking protein	-4.74e-01	6.83e-04	3.28e-02
SYF2	SYF2 pre-mRNA splicing factor	4.03e-01	6.70e-04	3.28e-02
RBBP8	RB binding protein 8, endonuclease	-6.72e-01	7.22e-04	3.39e-02
TMOD3	tropomodulin 3	5.35e-01	7.58e-04	3.50e-02
PSMB5	proteasome 20S subunit beta 5	2.83e-01	7.74e-04	3.52e-02
EXOSC7	exosome component 7	4.22e-01	7.91e-04	3.54e-02
EIF4E2	eukaryotic translation initiation factor 4E family member 2	4.56e-01	8.65e-04	3.67e-02
RBM8A	RNA binding motif protein 8A	2.51e-01	8.39e-04	3.67e-02
RPL6	ribosomal protein L6	6.04e-01	8.72e-04	3.67e-02
TUBG1	tubulin gamma 1	-3.70e-01	8.73e-04	3.67e-02
AK2	adenylate kinase 2	3.10e-01	9.03e-04	3.68e-02
RPS25	ribosomal protein S25	2.35e-01	9.01e-04	3.68e-02
MTCO3P12	MT-CO3 pseudogene 12	3.11e-01	9.24e-04	3.72e-02
H6PD	hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase	-6.16e-01	9.67e-04	3.73e-02
PTRH2	peptidyl-tRNA hydrolase 2	3.82e-01	9.61e-04	3.73e-02
YTHDC1	YTH domain containing 1	4.75e-01	9.41e-04	3.73e-02
CBWD1	COBW domain containing 1	4.46e-01	9.93e-04	3.77e-02
DDX39A	DExD-box helicase 39A	-4.38e-01	1.00e-03	3.77e-02
CBWD5	COBW domain containing 5	3.98e-01	1.04e-03	3.81e-02
PHLDA1	pleckstrin homology like domain family A member 1	-3.54e-01	1.03e-03	3.81e-02
MYL12B	myosin light chain 12B	2.22e-01	1.08e-03	3.89e-02
TP53	tumor protein p53	3.94e-01	1.10e-03	3.93e-02
MRPL37	mitochondrial ribosomal protein L37	-5.91e-01	1.14e-03	4.00e-02
ANKRD11	ankyrin repeat domain 11	3.42e-01	1.16e-03	4.05e-02
ZFX	zinc finger protein X-linked	5.14e-01	1.18e-03	4.06e-02
CEBPZ	CCAAT enhancer binding protein zeta	4.86e-01	1.24e-03	4.19e-02
ITGA3	integrin subunit alpha 3	-5.60e-01	1.28e-03	4.28e-02
RSRC2	arginine and serine rich coiled-coil 2	3.68e-01	1.29e-03	4.28e-02
SLU7	SLU7 homolog, splicing factor	5.34e-01	1.31e-03	4.30e-02
CAV1	caveolin 1	3.22e-01	1.38e-03	4.41e-02
TSG101	tumor susceptibility 101	-6.41e-01	1.37e-03	4.41e-02
MTND1P23	MT-ND1 pseudogene 23	2.55e-01	1.43e-03	4.42e-02
SDC1	syndecan 1	-5.31e-01	1.43e-03	4.42e-02
SEC61G	SEC61 translocon subunit gamma	2.42e-01	1.44e-03	4.42e-02
SNRPD1	small nuclear ribonucleoprotein D1 polypeptide	2.04e-01	1.41e-03	4.42e-02
PRPS2	phosphoribosyl pyrophosphate synthetase 2	-5.65e-01	1.48e-03	4.47e-02
DMAC2L	distal membrane arm assembly component 2 like	-6.54e-01	1.50e-03	4.50e-02
C15orf48	chromosome 15 open reading frame 48	-4.92e-01	1.54e-03	4.53e-02
MCM7	minichromosome maintenance complex component 7	-5.23e-01	1.54e-03	4.53e-02
LSM3	LSM3 homolog, U6 small nuclear RNA and mRNA degradation associated	2.50e-01	1.56e-03	4.54e-02
AK4	adenylate kinase 4	4.61e-01	1.65e-03	4.66e-02
DDX1	DEAD-box helicase 1	-4.15e-01	1.70e-03	4.66e-02
PIH1D1	PIH1 domain containing 1	-4.60e-01	1.69e-03	4.66e-02
RFXANK	regulatory factor X associated ankyrin containing protein	-6.05e-01	1.64e-03	4.66e-02
SARNP	SAP domain containing ribonucleoprotein	3.66e-01	1.66e-03	4.66e-02
SERPINE1	serpin family E member 1	-4.36e-01	1.70e-03	4.66e-02
UBXN4	UBX domain protein 4	-4.27e-01	1.80e-03	4.88e-02
PWP1	PWP1 homolog, endonuclein	-3.97e-01	1.82e-03	4.89e-02
COIL	coilin	4.82e-01	1.86e-03	4.94e-02

Gene Set Enrichment Analysis

There were too few differentially expressed genes to run a meaningful GSEA.

Literature Mining

INDRA was used to automatically assemble known mechanisms related to PRPF8 from literature and knowledge bases. The first section shows only DUB activity and the second shows all other results.

Deubiquitinase Activity

Other Statements

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PRPF8 affects SNRNP200

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PRPF8 inhibits SNRNP200.

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PRPF8 inhibits SNRNP200. 4 / 4

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Given the mechanistic analogy between histone mRNA processing and splicing, we thus envisage that SLBP ubiquitylation may suppress hPrp43 helicase activity in histone mRNA processing similar to Prp8 u[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]

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In humans, phosphorylation of Prp6 that occurs after the tri-snRNP being integrated into the B-complex may release the inhibition of Brr2 by Prp8 and is important for spliceosomal B-complex activation [ xref , xref ].

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The RNase H domain of PRPF8 inhibits loading of SNRNP200 to U4 snRNA, and a C-terminal part of PRPF8 modulates the SNRNP200 activity for the unwinding of U4/U6 snRNA duplex XREF_BIBR - XREF_BIBR.

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A model was proposed in which inhibition of Brr2 by the ubiquitinated Prp8 leads to spliceosome assembly and stabilization of the triple snRNP (U4/U6–U5).

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PRPF8 activates SNRNP200.

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PRPF8 activates SNRNP200. 2 / 2

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Although it has been demonstrated that the PRPF8 RNase H domain inhibits SNRNP200 activity and GTP bound EFTUD2 and that the C-terminus of PRPF8 induces the activation of SNRNP200 XREF_BIBR - XREF_BIBR, the detailed molecular mechanisms of the activation of SNRNP200 remains to be determined.

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The RNase H domain of PRPF8 inhibits loading of SNRNP200 to U4 snRNA, and a C-terminal part of PRPF8 modulates the SNRNP200 activity for the unwinding of U4/U6 snRNA duplex XREF_BIBR - XREF_BIBR.

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PRPF8 affects Cell Survival

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PRPF8 activates Cell Survival. 4 / 4

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Functionally , the loss of Prp8 may specifically inhibit cell viability and metastasis and activated EMT and PI3K / Akt in HCC cells .

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Upregulation of Prp8 promoted cell viability and migration in a human hepatic astrocyte line .

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The upregulation of Prp8 promoted cell viability , metastasis and the activity of the PI3K / Akt pathway in hepatic astrocytes cells and HCC cells .

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Upregulation of Prp8 promoted cell viability and migration in HCC cells .

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17alpha-ethynylestradiol affects PRPF8

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17alpha-ethynylestradiol increases the amount of PRPF8.

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17alpha-ethynylestradiol increases the amount of PRPF8. 3 / 3

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17alpha-ethynylestradiol decreases the amount of PRPF8.

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17alpha-ethynylestradiol decreases the amount of PRPF8. 1 / 1

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PRPF8 affects PLIN2

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PRPF8 activates PLIN2. 3 / 3

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PRPF8 C-terminal mutations cause an early onset and severe form of adRP, and structural analysis of this region shows that it inserts into the RNA binding tunnel of the SNRNP200 helicase.

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Nine mutations, six of which are novel, in the pre-mRNA splicing-factor genes PRPF3, PRPF8, and PRPF31, causing adRP have been identified in the Spanish population.

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Relevant to both these interpretations is the observation that adRP linked to chromosome 17p is caused by mutations in PRPC8 (McKie et al., 2001), the human ortholog of yeast splicing factor Prp8p (Br[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]

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PRPF8 affects cell population proliferation

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PRPF8 activates cell population proliferation.

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PRPF8 activates cell population proliferation. 2 / 2

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We show that PRPF8 lesions lead to neomorphic spliceosomal activity and increased cellular proliferation resulting in a distinct phenotype of aggressive myeloid malignancies with increased RS.

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( C ) The expression location of Prp8 was explored via immunofluorescence ( D ) CCK8 assay revealed that loss of Prp8 reduced cell proliferation .

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PRPF8 inhibits cell population proliferation.

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PRPF8 inhibits cell population proliferation. 1 / 1

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Unlike SF3B1, XREF_BIBR, XREF_BIBR PRPF8 knockdown leads to increased cellular proliferation and increased clonogenicity.

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PRPF8 affects ISPH

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PRPF8 inhibits ISPH.

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PRPF8 inhibits ISPH. 2 / 2

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Depletion of PRPF8 and treatment with a small molecule spliceosome inhibitor (PlaB) disrupts HDR, SSA, and end resection.

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Using two independent siRNAs targeting PRPF8 (siPRPF8-2, siPRPF8-4), as well as a previously described siRNA targeting XAB2 (siXAB2-4), we found that PRPF8 and XAB2 depletion cause a significantly greater decrease in the frequency of HDR and SSA, compared to EJ.

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PRPF8 activates ISPH.

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PRPF8 activates ISPH. 1 / 1

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We also found that transient expression of a myc tagged siRNA resistant PRPF8 rescued the HDR and SSA defects caused by siPRPF8-2 treatment.

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PRPF8 affects immune response

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PRPF8 activates immune response. 2 / 2

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Furthermore, PRPF8, AKAP9, UVRAG, HDAC10 and NFE2L1 are known to mediate the immune response.

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Furthermore , PRPF8 , AKAP9 , UVRAG , HDAC10 and NFE2L1 are known to mediate the immune response .

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PRPF8 affects cell death

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PRPF8 activates cell death. 2 / 2

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Knockdown of SF3B1, PRPF8, UBL5 and USP39 increased cell death in all cSCC cell lines.

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Knockdown of c-MYC attenuated cell death caused by depletion of PRPF8 or SF3B1 in SCCRDEBMet cells and depletion of PRPF8 in SCCRDEB4 cells.

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PRPF8 affects Neoplasm Metastasis

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PRPF8 activates Neoplasm Metastasis. 2 / 2

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Functionally , the loss of Prp8 may specifically inhibit cell viability and metastasis and activated EMT and PI3K / Akt in HCC cells .

32547101

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The upregulation of Prp8 promoted cell viability , metastasis and the activity of the PI3K / Akt pathway in hepatic astrocytes cells and HCC cells .

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PRPF8 affects Bloc1s3

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PRPF8 activates Bloc1s3. 2 / 2

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For example, a previous study suggested that mutations in PRPF8 may cause RP by disrupting the nuclear association of Brr2p with the U5 snRNP and, consequently, the assembly of the triple snRNP.

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Herein, we sought to correlate the retinal cell-type-specific disease phenotype with the splicing profile shown by a patient with autosomal recessive RP, caused by a mutation in pre-mRNA splicing factor 8 (PRPF8).

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PRPF8 affects ATPase activity

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PRPF8 inhibits ATPase activity. 2 / 2

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Interestingly, in contrast to its stimulation of Brr2 helicase activity, the wild-type Prp8 fragment actually inhibited the RNA-dependent ATPase activity of Brr2.

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WT Brr2 also demonstrates RNA stimulated ATPase activity which is inhibited by Prp8-CTR ( xref ).

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2,3,7,8-tetrachlorodibenzodioxine affects PRPF8

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2,3,7,8-tetrachlorodibenzodioxine increases the amount of PRPF8. 2 / 2

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Vinclozolin affects PRPF8

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Vinclozolin increases the amount of PRPF8.

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Vinclozolin increases the amount of PRPF8. 1 / 1

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Vinclozolin decreases the amount of PRPF8.

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Vinclozolin decreases the amount of PRPF8. 1 / 1

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Permethrin affects PRPF8

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Permethrin increases the amount of PRPF8.

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Permethrin increases the amount of PRPF8. 1 / 1

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Permethrin decreases the amount of PRPF8.

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Permethrin decreases the amount of PRPF8. 1 / 1

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PRPF8 affects RO60

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PRPF8 inhibits RO60.

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PRPF8 inhibits RO60. 1 / 1

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PRPF8 activates RO60.

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PRPF8 activates RO60. 1 / 1

| 1

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reach

We also found that transient expression of a myc tagged siRNA resistant PRPF8 rescued the HDR and SSA defects caused by siPRPF8-2 treatment.

29212152

Valproic acid affects PRPF8

1 |

Valproic acid decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

15901671

Tungsten affects PRPF8

1 |

Tungsten decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

30912803

Trimellitic anhydride affects PRPF8

1 |

Trimellitic anhydride increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19042947

Trichostatin A affects PRPF8

1 |

Trichostatin A decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

15901671

Thapsigargin affects PRPF8

1 |

Thapsigargin increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

24648495

Selenium atom affects PRPF8

1 |

Selenium atom increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19244175

Resveratrol affects PRPF8

1 |

Resveratrol decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

25505154

Phenobarbital affects PRPF8

1 |

Phenobarbital increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

31836555

Nickel dichloride affects PRPF8

1 |

Nickel dichloride increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19187980

Miconazole affects PRPF8

1 |

Miconazole increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

27462272

Methapyrilene affects PRPF8

1 |

Methapyrilene decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

30467583

Indometacin affects PRPF8

1 |

Indometacin decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

28628672

Hsa-miR-93-5p affects PRPF8

1 |

Hsa-miR-93-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-93-3p affects PRPF8

1 |

Hsa-miR-93-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-92a-3p affects PRPF8

1 |

Hsa-miR-92a-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-615-3p affects PRPF8

1 |

Hsa-miR-615-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-532-3p affects PRPF8

1 |

Hsa-miR-532-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-484 affects PRPF8

1 |

Hsa-miR-484 decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-409-3p affects PRPF8

1 |

Hsa-miR-409-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

24374217

Hsa-miR-381-3p affects PRPF8

1 |

Hsa-miR-381-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

24374217

Hsa-miR-361-5p affects PRPF8

1 |

Hsa-miR-361-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-324-5p affects PRPF8

1 |

Hsa-miR-324-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-296-3p affects PRPF8

1 |

Hsa-miR-296-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-27b-3p affects PRPF8

1 |

Hsa-miR-27b-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-25-3p affects PRPF8

1 |

Hsa-miR-25-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-218-5p affects PRPF8

1 |

Hsa-miR-218-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23212916

Hsa-miR-196a-5p affects PRPF8

1 |

Hsa-miR-196a-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-193b-3p affects PRPF8

1 |

Hsa-miR-193b-3p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-186-5p affects PRPF8

1 |

Hsa-miR-186-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-17-5p affects PRPF8

1 |

Hsa-miR-17-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-16-5p affects PRPF8

1 |

Hsa-miR-16-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-140-5p affects PRPF8

1 |

Hsa-miR-140-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-10b-5p affects PRPF8

1 |

Hsa-miR-10b-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-10a-5p affects PRPF8

1 |

Hsa-miR-10a-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-106a-5p affects PRPF8

1 |

Hsa-miR-106a-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-let-7e-5p affects PRPF8

1 |

Hsa-let-7e-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-let-7c-5p affects PRPF8

1 |

Hsa-let-7c-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-let-7b-5p affects PRPF8

1 |

Hsa-let-7b-5p decreases the amount of PRPF8. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hexabromocyclododecane affects PRPF8

1 |

Hexabromocyclododecane increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

29177809

Glyphosate affects PRPF8

1 |

Glyphosate decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

26302742

Gentamycin affects PRPF8

1 |

Gentamycin increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

22061828

Genistein affects PRPF8

1 |

Genistein decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

16705744

Fumonisin B1 affects PRPF8

1 |

Fumonisin B1 decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

16221962

Fluoranthene affects PRPF8

1 |

Fluoranthene decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

22406618

Disodium selenite affects PRPF8

1 |

Disodium selenite increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

18175754

Dicrotophos affects PRPF8

1 |

Dicrotophos increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

28302478

Diarsenic trioxide affects PRPF8

1 |

Diarsenic trioxide increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

15894607

Dexamethasone affects PRPF8

1 |

Dexamethasone decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

28628672

Crocidolite asbestos affects PRPF8

1 |

Crocidolite asbestos increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

29523930

Copper(II) chloride affects PRPF8

1 |

Copper(II) chloride decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19174923

Cisplatin affects PRPF8

1 |

Cisplatin decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

27594783

Cadmium dichloride affects PRPF8

1 |

Cadmium dichloride increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19187980

Bisphenol F affects PRPF8

1 |

Bisphenol F decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

28628672

Bisphenol A affects PRPF8

1 |

Bisphenol A increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

25181051

Benzo[a]pyrene affects PRPF8

1 |

Benzo[a]pyrene increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

22228805

Benzene affects PRPF8

1 |

Benzene increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

19162166

Ametryn affects PRPF8

1 |

Ametryn decreases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

29427468

All-trans-retinoic acid affects PRPF8

1 |

All-trans-retinoic acid increases the amount of PRPF8. 1 / 1

1 |

➶

ctd

No evidence text available

15894607

SNRNP200 affects PRPF8

| 1

SNRNP200 deubiquitinates PRPF8. 1 / 1

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reach

Following de-ubiquitination of Prp8, Brr2 is free to promote unwinding of U4/U6, resulting in activation of the spliceosome [48].

| PMC

Radiation, Ionizing affects PRPF8

| 1

Radiation, Ionizing activates PRPF8. 1 / 1

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reach

For localization studies of PRPF8 and XAB2, cells were treated with 10 Gy of IR, but allowed to recover for 30 min, and were treated with pre-extraction buffer (20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 300 mM sucrose, 0.25% Triton-X 100) just prior to fixation.

29212152

RRM1 affects PRPF8

| 1

RRM1 activates PRPF8. 1 / 1

| 1

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reach

PRPF8, SNRNP200, GTPBP4, SMCHD1, DHX15, PRPF6, A8KAP3, DHX9 and EPRS were significantly reduced by RRM1 or ZnF1 deletion with no trend of> 2-fold for any other deletion mutant (Supplemental Table S4).

29511296

PRPF8 affects mRNA processing

| 1

PRPF8 bound to 3D inhibits mRNA processing. 1 / 1

| 1

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reach

In the case of picornaviruses, it has been shown recently that the viral polymerase 3D binds to PRPF8, a core components of U5, and blocks mRNA maturation, with the consequent accumulation of the lariat intermediate [XREF_BIBR].

27575636

PRPF8 affects intein-mediated protein splicing

| 1

PRPF8 activates intein-mediated protein splicing. 1 / 1

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reach

The recent discovery of a small molecule inhibitor of the Prp8 intein that selectively inhibits splicing without targeting proteases and inhibits the growth of intein-containing C. gattii and C. neoformans is a particularly exciting breakthrough that suggests intein inhibitors may have therapeutic benefits (Li et al., 2021).

34692773

PRPF8 affects homologous recombination

| 1

PRPF8 inhibits homologous recombination. 1 / 1

| 1

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reach

Depletion of p53 binding protein 1 (53BP1) suppressed the HR defect induced by both BRCA1 loss and PRPF8 knockdown, suggesting that PRPF8 functions during HR at least in part via facilitating BRCA1 function.

| PMC

PRPF8 affects epithelial to mesenchymal transition

| 1

PRPF8 activates epithelial to mesenchymal transition. 1 / 1

| 1

➶

eidos

Prp8 activates EMT in HCC .

32547101

PRPF8 affects cellular component biogenesis

| 1

PRPF8 inhibits cellular component biogenesis. 1 / 1

| 1

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reach

However, this is not a general case because the Y2334N and F2314L mutations in PRPF8 do not block snRNP biogenesis while affecting splicing, similar to two RP mutations in the SNRNP200 gene.

28515276

PRPF8 affects cas9

| 1

PRPF8 activates cas9. 1 / 1

| 1

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reach

Fibroblasts from a patient with splicing factor retinitis pigmentosa caused by a missense mutation in the PRPF8 splicing factor were used to produce three diseased and three CRISPR and Cas9 corrected induced pluripotent stem cell (iPSC) clones.

30572641

PRPF8 affects TP53

| 1

PRPF8 inhibits TP53. 1 / 1

| 1

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reach

Mechanistically, loss of function mutations in zebrafishPRPF8 have been shown to result in missplicing of TP53, 36 while PRPF8 knockdown in mammalian cells led to increased compensatory transcriptional activity of TP53, 39 suggesting another possibility of synergetic interaction between PRPF8 defects and TP53.

24781015

PRPF8 affects RPS3

| 1

Mutated PRPF8 inhibits RPS3. 1 / 1

| 1

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reach

These RPE abnormalities are more severe in the homozygous Prpf3-T494M and Prpf8-H2309P mice, suggesting that RP18 and RP13 are caused by the gain-of-function or dominant negative effect of Prpf3 and Prpf8 mutations.

| PMC

PRPF8 affects RPL6B

| 1

Mutated PRPF8 inhibits RPL6B. 1 / 1

| 1

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reach

| PMC

PRPF8 affects RPE

| 1

PRPF8 activates RPE. 1 / 1

| 1

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reach

Graziotto et al. and Farkas et al. demonstrated that PRPF3, PRPF8 and PRPF31 mutations could cause dysfunction of RPE XREF_BIBR XREF_BIBR.

28216641

PRPF8 affects RHO

| 1

PRPF8 activates RHO. 1 / 1

| 1

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reach

It can be speculated that there is a convergent pathogenic mechanism caused by RHO haploinsufficiency, with a lack of rhodopsin due to an untranslated mutated protein and an insufficient RHO splicing rate caused by a mutation in PRPF8.

26321861

PRPF8 affects PRPF31

| 1

PRPF8 activates PRPF31. 1 / 1

| 1

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reach

Mutations of the splicing factor genes PRPF31, PRPF8, and HPRP3 cause RP11, 13, and 18, respectively (Baehr and Chen, 2001; Chakarova et al., 2002).

14659595

PRPF8 affects PRPF3

| 1

PRPF8 activates PRPF3. 1 / 1

| 1

➶

reach

The recent identification of mutations in human splicing factors, PRPF31 and PRPC8, led us to screen HPRP3 as a candidate in three chromosome 1q linked families.

11773002

PRPF8 affects MT-ATP6

| 1

PRPF8 activates MT-ATP6. 1 / 1

| 1

➶

eidos

Interestingly , while Snu114p / EFTUD2 interacts with both Brr2p / SNRNP200 and Prp8p / PRPF8 , EFTUD2 is associated with a craniofacial phenotype while SNRNP200 and PRPF8 both cause RP ( Figure 1 ) .

33584830

PRPF8 affects MCL1

| 1

PRPF8 increases the amount of MCL1. 1 / 1

| 1

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reach

Mcl1 protein expression was reduced by knockdown of UBL5, SART1 or PRPF8 in Mcl1 dependent cells (in contrast to PSMD14 knockdown that led to Mcl1 accumulation, XREF_FIG).

24556687