PAN2 Data Analysis

HGNC Gene Name
poly(A) specific ribonuclease subunit PAN2
HGNC Gene Symbol
PAN2
Identifiers
hgnc:20074 NCBIGene:9924 uniprot:Q504Q3
Orthologs
mgi:1918984 rgd:1303301
INDRA Statements
deubiquitinations all statements
Pathway Commons
Search for PAN2
Number of Papers
87 retrieved on 2022-05-22

DepMap Analysis

The Dependency Map (DepMap) is a genome-wide pooled CRISPR-Cas9 knockout proliferation screen conducted in more than 700 cancer cell lines spanning many different tumor lineages. Each cell line in the DepMap contains a unique barcode, and each gene knockout is assigned a “dependency score” on a per cell-line basis which quantifies the rate of CRISPR-Cas9 guide drop. It has been found that proteins with similar DepMap scores across cell lines, a phenomenon known as co-dependent genes, have closely related biological functions. This can include activity in the same or parallel pathways or membership in the same protein complex or the same pathway.

We identified the strongest seven co-dependent genes (“Symbol”) for DUBs and ran GO enrichment analysis. We used Biogrid, IntAct, and Pathway Commons PPIDs, and the NURSA protein-protein interaction databases (PPIDs) to determine whether co-dependent genes interact with one another. The “Evidence” column contains the PPIDs in which the interaction appears as well as whether there is support for the association by an INDRA statement. As another approach to identify potential interactors, we looked at proteomics data from the Broad Institute's Cancer Cell Line Encyclopedia (CCLE) for proteins whose expression across ~375 cell lines strongly correlated with the abundance of each DUB; it has previously been observed that proteins in the same complex are frequently significantly co-expressed. The correlations and associated p-values in the CCLE proteomics dataset are provided. And, we determined whether co-dependent genes yield similar transcriptomic signatures in the Broad Institute's Connectivity Map (CMap). A CMap score greater than 90 is considered significantly similar.

DepMap Correlations

Symbol Name DepMap Correlation Evidence CCLE Correlation CCLE Z-score CCLE p-value (adj) CCLE Significant CMAP Score CMAP Type
PAN3 poly(A) specific ribonuclease subunit PAN3 0.335 BioGRID IntAct INDRA (14) Reactome (3) 0.42 2.22 6.44e-13
IKZF4 IKAROS family zinc finger 4 0.231 0.20 1.02 8.00e-03
MSRB3 methionine sulfoxide reductase B3 0.231 -0.09 -0.60 2.57e-01
DDIT3 DNA damage inducible transcript 3 0.227 -0.15 -0.93 1.22e-01
APOF apolipoprotein F 0.216
ATP6AP1 ATPase H+ transporting accessory protein 1 -0.211 -0.30 -1.76 2.79e-07
ARHGAP9 Rho GTPase activating protein 9 0.209 0.46 2.43 1.01e-07

Dependency GO Term Enrichment

Gene set enrichment analysis was done on the genes correlated with PAN2using the terms from Gene Ontology and gene sets derived from the Gene Ontology Annotations database via MSigDB.

Using the biological processes and other Gene Ontology terms from well characterized DUBs as a positive control, several gene set enrichment analyses were considered. Threshold-less methods like GSEA had relatively poor results. Over-representation analysis with a threshold of of the top 7 highest absolute value Dependency Map correlations yielded the best results and is reported below.

GO Identifier GO Name GO Type p-value p-value (adj.) q-value

Literature Mining

INDRA was used to automatically assemble known mechanisms related to PAN2 from literature and knowledge bases. The first section shows only DUB activity and the second shows all other results.

Deubiquitinase Activity

psp cbn pc bel_lc signor biogrid lincs_drug tas hprd trrust ctd vhn pe drugbank omnipath conib crog dgi | rlimsp isi tees geneways eidos trips medscan sparser reach
PAN2 deubiquitinates ASF1A. 5 / 5
| 4

reach
Since ASF1A is reported as a ubiquitinated protein 49, we then asked whether USP52 functions to deubiquitinate ASF1A.

ubibrowser
Additionally, we find that USP52 is overexpressed in breast carcinomas, and its level of expression correlates with that of ASF1A.

reach
Interestingly, we demonstrate that USP52 is a bona fide ubiquitin specific protease, and USP52 promotes ASF1A deubiquitination and stabilization.

reach
USP52 deubiquitinates ASF1A.

reach
Nevertheless, our study identified that USP52 is a bona fide USP, and revealed that USP52 deubiquitinates and stabilizes histone chaperone ASF1A.

Other Statements

psp cbn pc bel_lc signor biogrid lincs_drug tas hprd trrust ctd vhn pe drugbank omnipath conib crog dgi | rlimsp isi tees geneways eidos trips medscan sparser reach
PAN2 affects ASF1A
| 17
PAN2 activates ASF1A.
| 15
PAN2 activates ASF1A. 10 / 15
| 15

reach
These results are in favor of the role of USP52 promoted ASF1A stabilization in chromatin assembly thus cell cycle progression, and indicate that USP52 and ASF1A axis is required for breast cancer cell proliferation.

reach
To corroborate the role of USP52 promoted ASF1A stabilization in chromatin replication, cellular extracts from cells used in MNase digestion assays were collected and analyzed by western blotting.

reach
USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

reach
Considering that ASF1A also plays an important role in replication independent chromatin assembly and chromatin disassembly during transcriptional activation XREF_BIBR, XREF_BIBR, XREF_BIBR, it is interesting to investigate whether USP52 promoted ASF1A stabilization plays a role in these processes.

reach
USP52 promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression.

reach
Collectively, these results point a role of the USP52 promoted ASF1A stabilization in promoting breast carcinogenesis.

reach
These results favor the argument that USP52 promoted ASF1A stabilization regulates DNA synthesis coupled chromatin assembly through ASF1A mediated deposition of H3K56Ac.

reach
Collectively, these observations suggest that USP52 promoted ASF1A stabilization is potentially associated with breast carcinogenesis.

reach
In this study, we revealed that the expression level of USP52 and ASF1A is upregulated in breast cancer and positively correlates with each other, and, indeed, USP52 promoted ASF1A stabilization is required for breast cancer cells to combat with genotoxic insults.

reach
Taken together, these data indicate that USP52 targets ASF1A and K129 for deubiquitination and USP52 is a bona fide deubiquitinase for ASF1A.
PAN2 inhibits ASF1A.
| 1
PAN2 inhibits ASF1A. 1 / 1
| 1

reach
Moreover, we reveal that impairment of USP52 promoted ASF1A stabilization results in growth arrest of breast cancer cells and sensitizes these cells to DNA damage.
PAN2 increases the amount of ASF1A.
| 1
Modified PAN2 increases the amount of ASF1A. 1 / 1
| 1

reach
Next, we found overexpression of USP52 was able to restore the expression of ASF1A in USP52 deficient cells.
PAN2 affects HIF1A
| 1 9
PAN2 activates HIF1A.
| 1 6
PAN2 activates HIF1A. 7 / 7
| 1 6

reach
To rule out any effects of USP52 on HIF1A translation, we performed polysome profiling on U2OS cells treated with either NT or USP52 siRNA.

reach
To investigate further the role of USP52in the HIF1A mediated hypoxic response, U2OS cells were treated with individual USP52 siRNA duplexes 1 and 3 and exposed to hypoxia.

reach
This demonstrates that USP52 knockdown specifically reduces HIF1A mRNA abundance, and supports the previous observation that decreased HIF1A mRNA is associated with up-regulation of HIF2A [XREF_BIBR].

reach
To obtain insight into the observed USP52 mediated stabilization of HIF1A mRNA, we performed proteomic analysis of USP52 immunoprecipitates to identify new USP52 associated proteins.

reach
Disrupting USP52 containing P-bodies by GW182 depletion reduces HIF1A mRNA.

eidos
USP52 increases HIF-1alpha protein levels through stabilizing HIF-1alpha mRNA instead of affecting HIF-1alpha ubiquitination [ 69,84 ] .

reach
During a screening of mediators for HIF1A mediated hypoxic response, USP52 and PAN2, a component of P-bodies, was found to promote the stability of HIF1A mRNA in 3 ' UTR dependent manner, as documented with the use of knockdown experiments.156 However, the direct binding of this protein to AREs was not studied, and interactions with de facto RPBs, such as TTP, can possibly happen in P-bodies.
PAN2 inhibits HIF1A.
| 2
PAN2 inhibits HIF1A. 2 / 3
| 2

reach
Depletion of USP52 reduced HIF1A mRNA and protein levels and resulted in reduced expression of HIF1A regulated hypoxic targets due to a 3 '-UTR (untranslated region)-dependent poly (A)-tail-length-independent destabilization in HIF1A mRNA.

reach
To address whether USP52 negatively regulates HIF1A protein through interactions with the VHL complex, U2OS cells were treated with MLN4924 [XREF_BIBR] to inhibit the NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8)-interacting enzyme and block VHL activity.
PAN2 increases the amount of HIF1A.
| 1
PAN2 increases the amount of HIF1A. 1 / 3
| 1

reach
USP52 increases HIF-1alpha protein levels through stabilizing HIF-1alpha mRNA instead of affecting HIF-1alpha ubiquitination [XREF_BIBR, XREF_BIBR].
TFDP1 affects PAN2
8 |
TFDP1 decreases the amount of PAN2. 8 / 8
8 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
E2F1 affects PAN2
7 |
E2F1 decreases the amount of PAN2. 7 / 7
7 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
Pbp1 affects PAN2
| 1 4
Pbp1 inhibits PAN2.
| 1 3
Pbp1 inhibits PAN2. 4 / 4
| 1 3

reach
Pbp1 negatively regulates Pan2 activity in the absence of Ccr4 in the stationary phase.

sparser
Further analysis should be needed to elucidate the physiological role of Pan2 inhibition by Pbp1 in the stationary phase.

reach
If Pan2 activity is inhibited by Pbp1, the LRG1 poly (A) tail length in the ccr4Delta pbp1Delta pan2Delta triple mutant would be longer than that in the ccr4Delta pbp1Delta double mutant in the stationary phase.

reach
Consistently, we found that the shortening of the LRG1 poly (A) tail length in the ccr4Delta pbp1Delta mutant required Pan2 deadenylase in vivo, and that Pbp1 inhibited Pan2 activity only in the stationary phase but not in the log phase.
Pbp1 activates PAN2.
| 1
Pbp1 activates PAN2. 1 / 1
| 1

reach
Thus, Pbp1 may enhance the translation in the absence of Ccr4 and Pan2 in an independent manner of the association of LRG1 mRNA to polysomes.
4 |
Valproic acid decreases the amount of PAN2. 4 / 4
4 |

ctd
No evidence text available

ctd
No evidence text available

ctd
No evidence text available

ctd
No evidence text available
| 4

reach
USP52 promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression.

reach
These results suggest that USP52 promotes chromatin assembly in an ASF1A dependent manner.

reach
USP52 promotes chromatin assembly through stabilizing ASF1A.

reach
We reveal that USP52 promotes chromatin assembly through stabilizing ASF1A, and point a role of USP52 in breast carcinogenesis and cellular resistance of breast cancer cells to DNA damage.
PAN3 affects PAN2
| 3
PAN3 inhibits PAN2.
| 1
PAN3 inhibits PAN2. 1 / 2
| 1

reach
It is therefore a possibility that PAN3 depletion prevents USP52 localization to P-bodies and thus contributes to the destabilization of AMD substrates, as we have observed in the case of HIF1A.
PAN3 activates PAN2.
| 2
PAN3 activates PAN2. 2 / 2
| 2

reach
Instead, Pan3 may modulate the activity of Pan2 poly (A) nuclease or link deadenylation to subsequent decay of the mRNA body.

reach
Because Pan3 is able to enhance localization of Pan2 to P-bodies (XREF_FIG), we then tested whether Pan3 also helps Ccr4 and Caf1 localize to P-bodies.
ASF1A affects PAN2
| 3
ASF1A activates PAN2. 3 / 3
| 3

reach
Meanwhile, western blotting analysis revealed that the upregulation of H3K56Ac trigged by IR was disrupted upon USP52 depletion, the effect of which could be restored by ASF1A gain of function.

reach
Indeed, USP52 deficiency impaired chromatin assembly and DNA replication as well as S-phase progression, the effect of which could be largely restored by ASF1A gain of function.

reach
Taken together, these data indicate that USP52 targets ASF1A and K129 for deubiquitination and USP52 is a bona fide deubiquitinase for ASF1A.
Guanosine affects PAN2
| 1 2
| 1 1

sparser
Caf1 shows strict specificity for poly(A) and is inhibited by all non-A nucleotides, whereas Pan2 is substantially inhibited only by guanosines at the end of a poly(A) tail ( xref ).

eidos
Caf1 shows strict specificity for poly ( A ) and is inhibited by all non-A nucleotides , whereas Pan2 is substantially inhibited only by guanosines at the end of a poly ( A ) tail ( Fig. 3A , B ) .
| 1

sparser
To understand why guanosines inhibit Pan2, we soaked AAGGA or AAGGAA RNAs into Pan2 UCH-Exo crystals, which diffracted to 3.3 Å resolution ( xref ).
Bisphenol A affects PAN2
3 |
Bisphenol A decreases the amount of PAN2.
2 |
Bisphenol A decreases the amount of PAN2. 2 / 2
2 |

ctd
No evidence text available

ctd
No evidence text available
Bisphenol A increases the amount of PAN2.
1 |
Bisphenol A increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
PAN2 affects NFkappaB
| 3
| 2

reach
PAN2 also suppressed NF-kappaB induction resulting from overexpression of several adapter proteins and protein kinases involved in the TNF or IL-1 receptor signal transduction, including TRAF2, TRAF6, RIP, IRAK2, and NF-kappaB-inducing kinase as well as the IkappaB kinases IKKalpha and IKKbeta.

reach
When expressed in HEK293 cells, PAN2 suppressed NF-kappaB induction by the cytokines tumor necrosis factor-alpha (TNFalpha) and interleukin-1beta (IL-1beta), suggesting that this protein operates at a point of convergence in these two cytokine signaling pathways.
PAN2 bound to CHUK inhibits NFkappaB. 1 / 1
| 1

reach
On the other hand, PAN2 binds IKKalpha to reduce tumor necrosis factor (TNF)-alpha and IL-1beta-related NF-kappaB activation.
PAN2 affects LRG1
| 3
PAN2 decreases the amount of LRG1.
| 2
Modified PAN2 decreases the amount of LRG1. 2 / 2
| 2

reach
However, the overexpression of PAN2 did not reduce Lrg1 level in the ccr4Delta background in the stationary phase (XREF_FIG).

reach
On the other hand, overexpression of PAN2 had little effect on LRG1 poly (A) tail length and did not reduce Lrg1 protein level in the ccr4Delta mutant.
PAN2 inhibits LRG1.
| 1
PAN2 inhibits LRG1. 1 / 1
| 1

reach
PAN2 overexpression partially decreased LRG1 poly (A) tail length (XREF_FIG, lane 3).
PABPC1 affects PAN2
| 3
PABPC1 activates PAN2.
| 2
PABPC1 activates PAN2. 2 / 2
| 2

reach
Because Pab1 stimulates Pan2 and interacts directly with Pan3 through its C-terminal region, an alternative interpretation could be that removal of the corresponding Pab1 domain inhibits deadenylation by preventing Pan2 action.

reach
Since Pab1 promotes Pan2 activity, one model is that this deadenylation reflects Pab1 bound to the first ~ 65 residues of the A tail, but the 3 ' most region is exposed and thereby rapidly deadenylated by Pan2.
PABPC1 inhibits PAN2.
| 1
PABPC1 inhibits PAN2. 1 / 1
| 1

reach
Because Pab1 stimulates Pan2 and interacts directly with Pan3 through its C-terminal region, an alternative interpretation could be that removal of the corresponding Pab1 domain inhibits deadenylation by preventing Pan2 action.
PABP affects PAN2
| 3
PABP activates PAN2. 2 / 2
| 2

reach
PABP regulates deadenylation of mRNPs by binding Pan3 and stimulating Pan2 nuclease activity and interacting with GW182 [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR] through a C-terminal motif that is removed by both viral proteinases.

reach
PAN2 is activated by PABP in vitro XREF_BIBR.
PABP bound to PAN3 activates PAN2. 1 / 1
| 1

reach
For example, PABP 's interaction with Pan3 enhances Pan2 nuclease activity.
Phenylmercury acetate decreases the amount of PAN2. 2 / 2
2 |

ctd
No evidence text available

ctd
No evidence text available
TFDP2 affects PAN2
2 |
TFDP2 decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
STAT5B affects PAN2
2 |
STAT5B decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
RELA affects PAN2
2 |
RELA decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
RB1 affects PAN2
2 |
RB1 decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available

reach
This study demonstrates that USP52 inhibits cancer cell proliferation through downregulation of cyclin D1 as well as AKT/mTOR signaling pathway inhibition.

reach
USP52 inhibits cell proliferation by stabilizing PTEN protein in non-small cell lung cancer.
HNF4A affects PAN2
2 |
HNF4A decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
FUS affects PAN2
| 2
FUS inhibits PAN2. 2 / 2
| 2

reach
These data suggest that FUS sequesters PAN2 on PABPC1 and prevents it from deadenylating the poly (A) tail of the bound mRNA (XREF_SUPPLEMENTARY).

reach
These data suggest that FUS might sequester PAN2 on the PABP from attacking the poly (A) tail.
E2F4 affects PAN2
2 |
E2F4 decreases the amount of PAN2. 2 / 2
2 |

biopax:msigdb
No evidence text available

biopax:msigdb
No evidence text available
2 |
Pirinixic acid increases the amount of PAN2.
1 |
Pirinixic acid increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Pirinixic acid activates PAN2.
1 |
1 |

ctd
No evidence text available
2 |
Benzo[a]pyrene increases the amount of PAN2.
1 |
Benzo[a]pyrene increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Benzo[a]pyrene decreases the amount of PAN2.
1 |
Benzo[a]pyrene decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
PAN2 affects translation
| 2
PAN2 inhibits translation.
| 1
PAN2 bound to PAN3 inhibits translation. 1 / 1
| 1

reach
In contrast, tethered PAN3 did not repress reporter expression (XREF_SUPPLEMENTARY), suggesting that the PAN2 and PAN3 complex can not repress translation in the absence of deadenylation.
PAN2 activates translation.
| 1
| 1

reach
To rule out any effects of USP52 on HIF1A translation, we performed polysome profiling on U2OS cells treated with either NT or USP52 siRNA.
Zinc atom affects PAN2
1 |
Zinc atom increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Uracil affects PAN2
| 1
Uracil inhibits PAN2. 1 / 1
| 1

eidos
The Stacked , Helical Structure of Poly ( A ) is Important in Deadenylation In vitro deadenylation assays showed that Pan2 was not strongly inhibited by uracils ( Us ) or cytosines ( Cs ) at the end of a poly ( A ) tail ( Fig. 3A , B ) , but these nucleotides did not show the characteristic CD signature of helical poly ( A ) , either alone ( Fig. 3E ) or in the context of oligo ( A ) ( Fig. 4A ) .
Tunicamycin affects PAN2
1 |
Tunicamycin increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Troglitazone affects PAN2
1 |
Troglitazone decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Topotecan affects PAN2
1 |
Topotecan decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Thapsigargin affects PAN2
1 |
Thapsigargin increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Tert-butyl hydroperoxide decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Quercetin affects PAN2
1 |
Quercetin increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |

ctd
No evidence text available
Paracetamol affects PAN2
1 |
Paracetamol decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Oxaliplatin affects PAN2
1 |
Oxaliplatin decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Methylmercury chloride decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Methyl methanesulfonate increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Lipopolysaccharide increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Jinfukang affects PAN2
1 |
Jinfukang increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Ionomycin affects PAN2
1 |
Ionomycin decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Hsa-miR-875-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hsa-miR-8055 affects PAN2
1 |
Hsa-miR-8055 decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-770-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6864-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6826-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6801-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6782-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6756-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-6726-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hsa-miR-6131 affects PAN2
1 |
Hsa-miR-6131 decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-5587-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hsa-miR-484 affects PAN2
1 |
Hsa-miR-484 decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4764-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-454-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-4446-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hsa-miR-4294 affects PAN2
1 |
Hsa-miR-4294 decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-377-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3679-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hsa-miR-3672 affects PAN2
1 |
Hsa-miR-3672 decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3663-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3155b decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3155a decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3144-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-3127-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-302d-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-302b-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-29a-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-222-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-129-5p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
1 |
Hsa-miR-1249-3p decreases the amount of PAN2. 1 / 1
1 |

biopax:mirtarbase
No evidence text available
Hexabromocyclododecane decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Formaldehyde affects PAN2
1 |
Formaldehyde increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Flutamide affects PAN2
1 |
Flutamide increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Endosulfan affects PAN2
1 |
Endosulfan decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Doxorubicin affects PAN2
1 |
Doxorubicin increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Dorsomorphin affects PAN2
1 |
Dorsomorphin decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Diarsenic trioxide decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Cytosine affects PAN2
| 1
| 1

eidos
The Stacked , Helical Structure of Poly ( A ) is Important in Deadenylation In vitro deadenylation assays showed that Pan2 was not strongly inhibited by uracils ( Us ) or cytosines ( Cs ) at the end of a poly ( A ) tail ( Fig. 3A , B ) , but these nucleotides did not show the characteristic CD signature of helical poly ( A ) , either alone ( Fig. 3E ) or in the context of oligo ( A ) ( Fig. 4A ) .
1 |
Cyclosporin A increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Coumestrol affects PAN2
1 |
Coumestrol increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Copper(II) sulfate decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Cobalt dichloride decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Carbon nanotube decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Butanal affects PAN2
1 |
Butanal decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Bis(2-ethylhexyl) phthalate decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Atrazine affects PAN2
1 |
Atrazine increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Abrine affects PAN2
1 |
Abrine increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Vehicle Emissions increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
VDR affects PAN2
1 |
VDR decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
TLX2 affects PAN2
1 |
TLX2 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
STAT5A affects PAN2
1 |
STAT5A decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
SRF affects PAN2
1 |
SRF decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
SP1 affects PAN2
1 |
SP1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
REL affects PAN2
1 |
REL decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
1 |
Plant Extracts increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Particulate Matter increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
PPARA affects PAN2
1 |
PPARA decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
POU3F1 affects PAN2
1 |
POU3F1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
PITX2 affects PAN2
1 |
PITX2 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
PCI 5002 affects PAN2
1 |
PCI 5002 increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
PAN2 affects transport
| 1
| 1

reach
An alternative view is that since a pan2 defect can block mRNA transport out of the nucleus in 50% of the cells observed, the combination of a ccr4 deletion and this transport defect is the cause for the lack of COX17 mRNA deadenylation in the ccr4 pan2 strain background.

reach
This study demonstrates that USP52 inhibits cancer cell proliferation through downregulation of cyclin D1 as well as AKT/mTOR signaling pathway inhibition.

reach
USP52 knockdown specifically impaired the hypoxia response, as it caused no such impairment to the NF-kappaB (nuclear factor kappaB) response after TNFalpha (tumour necrosis factor alpha) stimulation of the HeLa C57A cell line [XREF_BIBR].
| 1

reach
The paN2 allows Escherichia coli to liberate the sulfur of DBT and DBTs and pBADD produces a flavin reductase.
PAN2 affects cell cycle
| 1
| 1

reach
USP52 promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression.
PAN2 affects SLC2A1
| 1
PAN2 inhibits SLC2A1. 1 / 1
| 1

reach
Examination of GLUT1 and LDHA levels by immunoblot analysis of hypoxic U2OS cells revealed that USP52 depletion impairs the accumulation of GLUT1 and LDHA in response to hypoxia (XREF_FIG A), thus demonstrating that USP52 is required to potentiate the HIF1A mediated hypoxic response.
PAN2 affects PTEN
| 1
PAN2 inhibits PTEN. 1 / 1
| 1

reach
USP52 inhibits cell proliferation by stabilizing PTEN protein in non-small cell lung cancer.
PAN2 affects MTOR
| 1
PAN2 activates MTOR. 1 / 1
| 1

reach
This study demonstrates that USP52 inhibits cancer cell proliferation through downregulation of cyclin D1 as well as AKT/mTOR signaling pathway inhibition.
PAN2 affects LDHA
| 1
PAN2 inhibits LDHA. 1 / 1
| 1

reach
Examination of GLUT1 and LDHA levels by immunoblot analysis of hypoxic U2OS cells revealed that USP52 depletion impairs the accumulation of GLUT1 and LDHA in response to hypoxia (XREF_FIG A), thus demonstrating that USP52 is required to potentiate the HIF1A mediated hypoxic response.
PAN2 affects IKBKB
| 1
PAN2 inhibits IKBKB. 1 / 1
| 1

reach
PAN2 also suppressed NF-kappaB induction resulting from overexpression of several adapter proteins and protein kinases involved in the TNF or IL-1 receptor signal transduction, including TRAF2, TRAF6, RIP, IRAK2, and NF-kappaB-inducing kinase as well as the IkappaB kinases IKKalpha and IKKbeta.
PAN2 affects GRIA1
| 1
PAN2 inhibits GRIA1. 1 / 1
| 1

reach
Indeed, the knockdown of PAN2 increased the GluA1 protein and mature mRNA levels, while leaving the primary transcript level unchanged (XREF_SUPPLEMENTARY).
PAN2 affects DBT
| 1
PAN2 activates DBT. 1 / 1
| 1

reach
The paN2 allows Escherichia coli to liberate the sulfur of DBT and DBTs and pBADD produces a flavin reductase.
PAN2 affects AKT
| 1
PAN2 inhibits AKT. 1 / 1
| 1

reach
This study demonstrates that USP52 inhibits cancer cell proliferation through downregulation of cyclin D1 as well as AKT/mTOR signaling pathway inhibition.
NR3C1 affects PAN2
1 |
NR3C1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
NR2F2 affects PAN2
1 |
NR2F2 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
| 1

reach
Although the conserved cysteine residue in the triad of classical DUBs is replaced by alanine (A526) in the UCH domain of USP52, we demonstrated that addition of the alkylating reagent N-ethylmaleimide (NEM), a known inhibitor of cysteine proteases XREF_BIBR, XREF_BIBR, almost completely abolished the catalytic activity of USP52 against K48- or K63 linked tetra-ubiquitins.

reach
In order to exclude USP52 knockdown promoting HIF1A depletion through alternative degradation pathways, U2OS cells depleted of USP52 were treated with the proteasome inhibitor MG132.
MYB affects PAN2
1 |
MYB decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
L-thyroxine affects PAN2
| 1
| 1

reach
The PCR product from the Pan 2 reaction was treated with T4 DNA polymerase (Pharmacia) to blunt the ends, and then digested with BamHl.
HMGA1 affects PAN2
1 |
HMGA1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
HAND1 affects PAN2
1 |
HAND1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
GATA1 affects PAN2
1 |
GATA1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
EIF4E affects PAN2
| 1
EIF4E inhibits PAN2. 1 / 1
| 1

reach
Surprisingly, in temperature sensitive eIF4E strains, deadenylation of the Gal1 mRNA increases even in a ccr4Delta strain, which has been interpreted to suggest that eIF4E can also inhibit Pan2 based deadenylation.
E4F1 affects PAN2
1 |
E4F1 decreases the amount of PAN2. 1 / 1
1 |

biopax:msigdb
No evidence text available
CNOT7 affects PAN2
| 1
CNOT7 inhibits PAN2. 1 / 1
| 1

reach
PAN2 depletion, like CAF1 depletion, did not cause an increase in steady-state levels of these mRNAs (XREF_FIG and E, and data not shown).
1 |
C646 compound increases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
Aroclor 1254 affects PAN2
1 |
Aroclor 1254 decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available
1 |
Air Pollutants decreases the amount of PAN2. 1 / 1
1 |

ctd
No evidence text available