DUB Portal
Venn diagram showing overlaps between DUB Portal and FamPlex entries for DUBs

Deubiquitinating enzymes (DUBs) are proteases that remove ubiqutin conjugates from proteins, thereby regulating protein turnover. However, the majority of substrates and pathways regulated by DUBs remain unknown, impeding efforts to prioritize specific proteins for research and drug-development.

On this DUB portal, we provide the integration and analysis of publicly available resources as well as newly collected transcriptomic data following DUB knockout or inhibition to facilitate the exploration of DUB function in oncology.

Each DUB is listed with the number of papers in PubMed, the number of INDRA statements about deubiquitinating substrates, the number of INDRA statements total (including upstream and downstream processes beyond deubiquitination), and the and the fraction of cell lines in the DepMap that are strongly dependent on each DUB (i.e., cancer cell proliferation is strongly impacted by DUB knockout). Blank values in the "Dependent Cell Lines" column means the DUB was not knocked out in the DepMap dataset. Additionally, we list the Gene Ontology (GO) term most strongly enriched in the top seven co-dependent genes for each DUB and the corresponding p-values. Finally, the "DGEA" column contains the number of differentially expressed genes 96 hours following knockout of the particular DUB in MDAMB231 cells as measured by high-throughput RNAseq. Blank values in "DGEA" refer to DUBs that were not knockout out in the RNAseq experiment. Click on an individual DUB name for more details and additional data and analysis.

DUB DUB Name DUB Family # Papers # DUB Stmts. # Other Stmts. Dependent Cell Lines # Sig. ORA Gene Sets (DepMap) DGEA # Sig. GSEA Gene Sets (DGEA)
ATXN3 ataxin 3 MJD 734 16 626 1.0% 0
ATXN3L ataxin 3 like MJD 7 1 22 0.0% 0
BAP1 BRCA1 associated protein 1 UCHL 1329 18 356 76.0% 4 10
BRCC3 BRCA1/BRCA2-containing complex subunit 3 JAMM 82 22 174 0.0% 2 1
COPS5 COP9 signalosome subunit 5 JAMM 339 17 551 100.0% 7 52 4
COPS6 COP9 signalosome subunit 6 JAMM 46 5 144 100.0% 0
CYLD CYLD lysine 63 deubiquitinase USP 728 70 782 5.0% 41 28 259
EIF3F eukaryotic translation initiation factor 3 subunit F JAMM 71 2 141 98.0% 21
EIF3H eukaryotic translation initiation factor 3 subunit H JAMM 66 0 107 96.0% 16
JOSD1 Josephin domain containing 1 MJD 11 0 95 9.0% 0 0
JOSD2 Josephin domain containing 2 MJD 6 0 42 0 0
MINDY1 MINDY lysine 48 deubiquitinase 1 MINDY 6 0 79 23.0% 0
MINDY2 MINDY lysine 48 deubiquitinase 2 MINDY 4 0 77 49.0% 0
MINDY3 MINDY lysine 48 deubiquitinase 3 MINDY 2 0 70 0
MINDY4 MINDY lysine 48 deubiquitinase 4 MINDY 1 0 30 0
MINDY4B MINDY family member 4B MINDY 0 0 0 0
MPND MPN domain containing JAMM 11 0 29 5.0% 0 0
MYSM1 Myb like, SWIRM and MPN domains 1 JAMM 60 1 107 0 0
OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 OTU 126 21 274 3.0% 11 0
OTUB2 OTU deubiquitinase, ubiquitin aldehyde binding 2 OTU 27 8 146 0 0
OTUD1 OTU deubiquitinase 1 OTU 24 5 157 0 0
OTUD3 OTU deubiquitinase 3 OTU 21 2 81 1.0% 0
OTUD4 OTU deubiquitinase 4 OTU 23 1 183 0 0
OTUD5 OTU deubiquitinase 5 OTU 26 11 108 48.0% 11 2
OTUD6A OTU deubiquitinase 6A OTU 4 0 28 0.0% 0
OTUD6B OTU deubiquitinase 6B OTU 32 1 42 1.0% 0 9
OTUD7A OTU deubiquitinase 7A OTU 20 14 33 0 2
OTUD7B OTU deubiquitinase 7B OTU 60 15 250 0 0
OTULIN OTU deubiquitinase with linear linkage specificity OTU 82 4 134 1.0% 0
OTULINL OTU deubiquitinase with linear linkage specificity like OTU 2 0 102 0.1% 0
PAN2 poly(A) specific ribonuclease subunit PAN2 USP 84 1 140 7.0% 0 0
PRPF8 pre-mRNA processing factor 8 JAMM 127 0 111 100.0% 14 106
PSMD14 proteasome 26S subunit, non-ATPase 14 83 11 185 100.0% 57 839 913
PSMD7 proteasome 26S subunit, non-ATPase 7 Proteasome 33 0 138 100.0% 6
STAMBP STAM binding protein JAMM 62 15 244 32.0% 4 1
STAMBPL1 STAM binding protein like 1 JAMM 23 3 119 0 0
TNFAIP3 TNF alpha induced protein 3 OTU 1433 8 595 1.0% 17 36 159
UCHL1 ubiquitin C-terminal hydrolase L1 UCHL 1549 19 688 0 11
UCHL3 ubiquitin C-terminal hydrolase L3 UCHL 93 9 183 1.0% 33 77 393
UCHL5 ubiquitin C-terminal hydrolase L5 UCHL 150 21 229 50.0% 8 3
USP1 ubiquitin specific peptidase 1 USP 191 28 350 14.0% 7 0
USP10 ubiquitin specific peptidase 10 USP 170 34 265 69.0% 0 1
USP11 ubiquitin specific peptidase 11 USP 88 38 191 0 0
USP12 ubiquitin specific peptidase 12 USP 53 11 136 0 1
USP13 ubiquitin specific peptidase 13 USP 73 19 241 1.0% 0 2
USP14 ubiquitin specific peptidase 14 USP 266 21 376 9.0% 1 2
USP15 ubiquitin specific peptidase 15 USP 141 54 384 1.0% 1 2
USP16 ubiquitin specific peptidase 16 USP 57 2 114 1.0% 0 10
USP17L1 ubiquitin specific peptidase 17 like family member 1 USP 0 0 3 0
USP17L2 ubiquitin specific peptidase 17 like family member 2 USP 42 19 106 0 0
USP18 ubiquitin specific peptidase 18 USP 285 10 422 16.0% 7 9
USP19 ubiquitin specific peptidase 19 USP 60 8 99 34.0% 0 10
USP2 ubiquitin specific peptidase 2 USP 187 21 326 1.0% 0 14
USP20 ubiquitin specific peptidase 20 USP 42 15 80 0 14
USP21 ubiquitin specific peptidase 21 USP 68 4 43 3.0% 0 1
USP22 ubiquitin specific peptidase 22 USP 198 26 383 1.0% 22 2
USP24 ubiquitin specific peptidase 24 USP 30 6 154 4.0% 0 10
USP25 ubiquitin specific peptidase 25 USP 67 40 293 0 0
USP26 ubiquitin specific peptidase 26 USP 54 4 54 0 2
USP27X ubiquitin specific peptidase 27 X-linked USP 15 8 45 0 1
USP28 ubiquitin specific peptidase 28 USP 105 19 234 1.0% 62 9
USP29 ubiquitin specific peptidase 29 USP 45 1 29 0 9
USP3 ubiquitin specific peptidase 3 USP 52 7 153 0.0% 0 15
USP30 ubiquitin specific peptidase 30 USP 63 3 64 0.0% 0 3
USP31 ubiquitin specific peptidase 31 USP 13 0 71 0 5
USP32 ubiquitin specific peptidase 32 USP -1 0 92 2.0% 10 0
USP33 ubiquitin specific peptidase 33 USP 59 18 119 1.0% 0 0
USP34 ubiquitin specific peptidase 34 USP 42 5 114 1.0% 0 11
USP35 ubiquitin specific peptidase 35 USP 19 4 49 6.0% 2 0
USP36 ubiquitin specific peptidase 36 USP 48 8 103 100.0% 2 3
USP37 ubiquitin specific peptidase 37 USP 39 13 182 80.0% 3 2
USP38 ubiquitin specific peptidase 38 USP 19 0 65 2.0% 47 3
USP39 ubiquitin specific peptidase 39 USP 54 2 80 67.0% 0 10
USP4 ubiquitin specific peptidase 4 USP 138 42 236 6.0% 0 1
USP40 ubiquitin specific peptidase 40 USP 14 0 43 0 0
USP41 ubiquitin specific peptidase 41 USP 5 0 14 0
USP42 ubiquitin specific peptidase 42 USP 25 2 87 1.0% 10 0
USP43 ubiquitin specific peptidase 43 USP 6 0 46 0 0
USP44 ubiquitin specific peptidase 44 USP 46 10 105 1.0% 0 0
USP45 ubiquitin specific peptidase 45 USP 101 3 68 0 0
USP46 ubiquitin specific peptidase 46 USP 53 14 152 0 6
USP47 ubiquitin specific peptidase 47 USP 40 4 132 0.0% 0 0
USP48 ubiquitin specific peptidase 48 USP 39 4 166 1.0% 0 1
USP49 ubiquitin specific peptidase 49 USP 23 5 113 0 0
USP5 ubiquitin specific peptidase 5 USP 107 10 225 100.0% 4 1
USP50 ubiquitin specific peptidase 50 USP 5 4 15 2.0% 0 2
USP51 ubiquitin specific peptidase 51 USP 15 5 51 0.0% 1 0
USP53 ubiquitin specific peptidase 53 USP 18 1 111 2.0% 2 0
USP54 ubiquitin specific peptidase 54 USP 9 0 56 0 0
USP6 ubiquitin specific peptidase 6 USP 185 2 81 14.0% 0 11
USP7 ubiquitin specific peptidase 7 USP 588 102 575 81.0% 47 16
USP8 ubiquitin specific peptidase 8 USP 243 61 260 98.0% 18 69 359
USP9X ubiquitin specific peptidase 9 X-linked USP 297 57 597 92.0% 3 2
USP9Y ubiquitin specific peptidase 9 Y-linked USP 89 0 17 0 2
USPL1 ubiquitin specific peptidase like 1 USP 7 0 74 99.0% 4 813 113
VCPIP1 valosin containing protein interacting protein 1 OTU 18 3 93 35.0% 3 0
YOD1 YOD1 deubiquitinase OTU 44 8 283 0 21 155
ZRANB1 zinc finger RANBP2-type containing 1 OTU 24 5 146 2.0% 0 30 327
ZUP1 zinc finger containing ubiquitin peptidase 1 7 18 142 0